Table 2 Primer pairs used for the experimental methods
From: Novel mutations of the SRF gene in Chinese sporadic conotruncal heart defect patients
| Primers | Forward (5′ → 3′) | Reverse (5′ → 3′) |
|---|---|---|
| SRF-seq | TGCCAGGTAGTGTTTTCTAAGTG | GGCCCCTATTCACCTTCCTT |
| Mut-G274D | CACCAACCTGCCGGATACAACCTCCACCA | TGGTGGAGGTTGTATCCGGCAGGTTGGTG |
| Mut-G294C | GCAAGTCAGCAGCTGCCCCTCCTTTCC | GGAAAGGAGGGGCAGCTGCTGACTTGC |
| RT-SRF | ACTCTCCACCCCGTTCAGAC | TGGTGCACTTGAATGGCCTG |
| GAPDH | GAGTCAACGGATTTGGTCGT | TGATTTTGGAGGGATCTCG |