The mutational spectrum of hunter syndrome reveals correlation between biochemical and clinical profiles in Tunisian patients

Table 1 Primers for PCR and DNA sequencing for detection of IDS mutations

Primer	Sequence 5′ > 3′	Tm (°C)	Expected products (bp)
F1-IDS	GAGGAGGTCTCTGTGGCTGC	63 .5	376
R1-IDS	AGGGACGGTAGGAAGGAGTG	61 .4	376
F2-IDS	CACTCACTATCTCGCTTCCTC	59.8	540
R2-IDS	CCTCTAACAAGATGTCCCG	56.7	540
F3-IDS	GGTTACCTAAGAGATGGCAG	57.3	542
R3-IDS	CAGCCTGTGTCCTCCCTAC	61.0	542
F4-IDS	GTAGATGAGGAAACTGAGCC	57.3	475
R4-IDS	CTATTCAATGAGTCTGACACG	55.9	475
F5-IDS	GCCTGGAAAACAAGAAACACC	57.9	487
R5-IDS	TGGCGATGGCAGGATGTAG	58.8	487
F6-IDS	AGGCAGGAGGTGGGGACAG	63.1	607
R6-IDS	CCAGCACTTTGCCTGATAACTC	60.3	607
F7-IDS	CTAAGGGGTAGGGATTGGGAG	61.8	440
R7-IDS	ACCCACACCTATCCGTCAAGC	61.8	440
F8-IDS	GGTGATGAGTTTCTACTTCCT	55.9	465
R8-IDS	GAGATGTTCAGAAAGCGTG	54.5	465
F9-IDS	GTGAGGTGCCGAGGTGGTG	63.1	468
R9-IDS	GGTGCGTATGGAATAGCCC	58.8	468
F9–1-IDS	CTTCAGACATCCCTCAGTGG	95.4	283
R9–1-IDS	GCTCTAACTCCTCCTCTCACC	61.8	283

ISSN: 1471-2350