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Table 1 PCR protocol

From: Identification of ATP2C1 mutations in the patients of Hailey-Hailey disease

94 °C 5 min
I94 °C 45 s60 °C 45 s72 °C 45 s2×1
94 °C 45 s59 °C 45 s72 °C 45 s2×
94 °C 45 s58 °C 45 s72 °C 45 s2×
94 °C 45 s57 °C 45 s72 °C 45 s2×
94 °C 45 s56 °C 45 s72 °C 45 s2×
94 °C 45 s55 °C 45 s72 °C 45 s20×
II94 °C 45 s55 °C 45 s72 °C 45 s32×Other exon
III94 °C 30s54 °C 30s72 °C 30s30×5, 7, 12, 27, 28
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BMC Medical Genetics

ISSN: 1471-2350

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